Journal: Nature Communications

Article Title: Prefrontal cortex molecular clock modulates development of depression-like phenotype and rapid antidepressant response in mice

doi: 10.1038/s41467-024-51716-9

Figure Lengend Snippet: a , b Representative western blots and quantitative data of synaptic AMPA receptors subunits GluA1 and GluA2 levels normalized to Homer1b/c and PSD95 in mPFC of WT mice 24 h after SR10067 application ( a ), and 6 h and 24 h post SR1078 injection ( b ). ( n = 5, a : two-tailed Student’s t test: * P = 0.0422 for GluA1, ** P = 0.0069 for GluA2, b : one-way ANOVA with Bonferroni post hoc test: * P < 0.05, *** P < 0.001). c Experimental design: WT mice were subjected to the CDM protocol before undergoing implantation of ECoG, LFP and EMG recording electrodes. After recovery, mice were recorded in their home cage over 32 h from ZT0, with treatment (vehicle/ketamine/SR1078) administered at ZT06. d , e Delta power (0.5–4 Hz) of LFP signal recorded from the mPFC ( d ) and ECoG signal ( e ) recorded during slow wave sleep (SWS) across 12 h:12 h LD conditions ( n = 14 mice CDM/vehicle; n = 6 SR1078; n = 4 KET; repeated measures mixed-effects model two-way ANOVA with Bonferroni post hoc test * P < 0.05, ** P < 0.01 vs CDM/vehicle). Black arrow indicates time of treatment administration. f Schema summarizing the effects of RORα/γ activation on BMAL1, Homer1a and synaptic AMPAR expression in the mPFC and the relation to depression-like behavior. Data are presented as mean ± SEM and the individual data points are depicted. See also Supplementary Fig. and Supplementary Data . Source data are provided as a Source Data file.

Article Snippet: The membranes were blocked with 5% non-fat dry milk in TBS-T (1% Tween20 in Tris-buffered saline (TBS)) and afterward incubated with the respective primary antibodies diluted in TBS (mouse anti-GluR1-NT, Merck (MAB2263), 1:1000; rabbit anti-GluR2, Merck (AB1768-I), 1:1000; rabbit anti-Homer1, Merck (ABN37), 1:1000; mouse anti-PSD95, Merck (MABN68), 1:1000; rabbit anti-mouse Per2, Alpha Diagnostics (PER21-A), 1:1000; rabbit anti-BMAL1, Thermo Fisher Scientific PA1-523, 1:1000; mouse anti-Histone H2b, Euromedex (IG-H2-2A8), 1:500 overnight at 4 °C.

Techniques: Western Blot, Injection, Two Tailed Test, Activation Assay, Expressing